Computational protocol: OsCNGC13 promotes seed-setting rate by facilitating pollen tube growth in stylar tissues

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Protocol publication

[…] Gene prediction was performed using the Rice Genome Automated Annotation System (http://ricegaas.dna.affrc.go.jp/). Sequence analysis of OsCNGC13 and OsCNGC13-D was performed using the SOSUI program (http://bp.nuap.nagoyau.ac.jp/sosui/). Amino acid sequences of OsCNGC13 and OsCNGC13-D were used for alignment using the ClustalX 2.01 program with default settings. The BioEdit software was also used to perform multiple sequence alignments to confirm the ClustalX data output. […]

Pipeline specifications

Software tools SOSUI, Clustal W, BioEdit
Databases RiceGAAS
Application Membrane protein analysis
Chemicals Calcium, Nucleotides