Dataset features

Specifications


Dataset type: Expression profiling by high throughput sequencing
Number of samples: 1922
Release date: Dec 31 2018
Last update date: Feb 19 2019
Access: Public
Dataset link Deep single-cell RNAseq of microglia and brain myeloid cells from various brain regions and developmental stages

Experimental Protocol


Single microglia or myeloid cells from a given time point and region were FACS sorted from pooled male animal samples (C57BL/6N) into 96-well plates. Libraries were prepared with a semi-automated Smart-seq2 protocol. Each Nextseq sequencing run was done by pooling ~350 cells with the unique Illumina 384 indexes. Three QC criteria were used (Y=passed, N=not passed), and only cells that passed all three criteria were used for downstream analysis.

Repositories


GEO

GSE123025

ENA

SRP170969

BioProject

PRJNA507279

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Contact


Qingyun Li