|Dataset type:||Expression profiling by high throughput sequencing|
|Number of samples:||1922|
|Release date:||Dec 31 2018|
|Last update date:||Feb 19 2019|
|Dataset link||Deep single-cell RNAseq of microglia and brain myeloid cells from various brain regions and developmental stages|
Single microglia or myeloid cells from a given time point and region were FACS sorted from pooled male animal samples (C57BL/6N) into 96-well plates. Libraries were prepared with a semi-automated Smart-seq2 protocol. Each Nextseq sequencing run was done by pooling ~350 cells with the unique Illumina 384 indexes. Three QC criteria were used (Y=passed, N=not passed), and only cells that passed all three criteria were used for downstream analysis.