Computational protocol: Transglutaminase-Mediated Semen Coagulation Controls Sperm Storage in the Malaria Mosquito

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Protocol publication

[…] The selected amino acid sequences were subjected to multiple alignments using the Clustal W (http://www.ebi.ac.uk/Tools/clustalw/) and Clustal X (1.83) algorithms. A phylogenetic tree was constructed by the neighbour joining method using p-distance estimates, tested by the interior-branch test, and displayed using TreeView (1.6.6) software. Reliability of each node was assessed with 1,000 bootstrap replications. The genomic locations of the TGase genes encoding the proteins used in the tree, as well as Plugin, are reported in . […]

Pipeline specifications

Software tools Clustal W, TreeViewX
Application Phylogenetics
Organisms Anopheles gambiae