|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Mar 30 2016|
|Last update date:||Jul 19 2017|
|Dataset link||s-SHIP expression identifies a subset of murine basal prostate cells as neonatal stem cells|
Prostate tissue was collected from 6-day-old male mice, minced into small fragment, digested with 200 U/ml collagenase IA–S (Sigma; C5894) in Dulbecco’s modified Eagles medium (DME, Gibco) supplemented with 10% fetal bovine serum (FBS, Hyclone) (DME-10% FBS) at 37°C for 60 min with gentle agitation. The digested cells were filtered through a 40-μm cell strainer (BD Biosciences) washed, and resuspended in DME-10% FBS., filtered and labeled with antibodies against lineage (Ter119, CD31, CD45), CD49f and Sca-1 cell surface markers (Affymetrix ebiosciences). Labeled cells were analyzed and the GFP-negative LSC and GFP-positive-LSC populations were sorted by FACS. For each cell population, 3 independent samples were collected and analysed.
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