Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Mar 30 2016
Last update date: Jul 19 2017
Access: Public
Dataset link s-SHIP expression identifies a subset of murine basal prostate cells as neonatal stem cells

Experimental Protocol


Prostate tissue was collected from 6-day-old male mice, minced into small fragment, digested with 200 U/ml collagenase IA–S (Sigma; C5894) in Dulbecco’s modified Eagles medium (DME, Gibco) supplemented with 10% fetal bovine serum (FBS, Hyclone) (DME-10% FBS) at 37°C for 60 min with gentle agitation. The digested cells were filtered through a 40-μm cell strainer (BD Biosciences) washed, and resuspended in DME-10% FBS., filtered and labeled with antibodies against lineage (Ter119, CD31, CD45), CD49f and Sca-1 cell surface markers (Affymetrix ebiosciences). Labeled cells were analyzed and the GFP-negative LSC and GFP-positive-LSC populations were sorted by FACS. For each cell population, 3 independent samples were collected and analysed.

Repositories


GEO

GSE73758

ArrayExpress

E-GEOD-73758

BioProject

PRJNA297832

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Contact


frédéric leprêtre

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