Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Jun 30 2016
Last update date: Jul 3 2016
Access: Public
Diseases: Parkinson Disease
Chemicals: Dopamine
Dataset link Expression mRNA data from human postmortem putamen samples measured using the NanoString nCounter platform

Experimental Protocol


Human postmortem putamen tissues from 12 patients with PD symptoms and 12 neurologically normal controls were used in the study. Samples were obtained from the Human Brain and Spinal Fluid Resource Center, Los Angeles, CA through NIH NeuroBioBank, and stored at -80°C until RNA isolation. Total RNA was extracted using the mirVana RNA isolation kit, following the manufacturer’s instructions (Ambion). Using 225ng total RNA, mRNA levels were assayed by direct digital detection using Nanostring nCounter assay kits (Nanostring Technologies).

Repositories


GEO

GSE77666

ArrayExpress

E-GEOD-77666

BioProject

PRJNA311175

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Contact


Venugopalan Nair