Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Jun 21 2014
Last update date: Jul 26 2018
Access: Public
Diseases: Breast Neoplasms
Chemicals: Estrogens, Tretinoin
Dataset link Expression data for Control and SMRT-Depleted MCF-7 Cells

Experimental Protocol


We sought to determine the genes that are regulated by depletion of the coregulator SMRT using Affymetrix Human Gene 1.0 ST Array. To this end, we transfected MCF-7 cells with control siRNA or SMRT-targeting siRNA for 48 h and treated for an additional 4 or 24 h with vehicle (0.1% EtOH) or 1 nM estradiol (E2). A total of 24 samples were analyzed, separated into eight groups each with three experimental replicates in each group, siControl-Veh 4 h, siControl -E2 4 h, siSMRT-Veh 4 h, siSMRT-E2 4 h, siControl-Veh 24 h, siControl-E2 24 h, siSMRT-Veh 24 h, siSMRT-E2 24 h.

Repositories


GEO

GSE57935

ArrayExpress

E-GEOD-57935

BioProject

PRJNA251372

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Contact


Carolyn Smith
Carolyn L Smith