Computational protocol: Inter-individual diversity and intra-individual stability of amphetamine-induced sensitization of frequency-modulated 50-kHz vocalization in Sprague–Dawley rats

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Protocol publication

[…] The rats were brought in their home cages to the testing room, placed singly in fresh opaque plastic cages (56 × 34 cm floor size) with no bedding, and immediately tested for locomotor activity (distance covered) for 15 min using a model YR-600 1/3 in. CCD 540 TVL camera (Sony, Japan) fixed to the room ceiling and a PC equipped with the EthoVision® XT Video Tracking System v. 7 (Noldus Information Technology B.V., Wageningen, The Netherlands). [...] Data are expressed as the mean ± SEM except when specified otherwise or shown individually. USV rate data (numbers of FM 50-kHz calls/time unit) were analyzed by a two-way or three-way ANOVA as needed, followed by post-hoc Tukey’s test when appropriate, except that “anticipatory” USV rate data, because of extreme deviation from normal distribution, were analyzed using nonparametric tests (Friedman’s ANOVA or Kruskal–Wallis ANOVA followed by the Dunn test of multiple comparisons when appropriate). For the analysis of predictive power of the various tests utilized, the rats were classified into low and high responders using “median split”. In all cases, a p < 0.05 was considered significant. All statistical analyses were performed using the Statistica v. 7.1 software package (StatSoft Inc., Tulsa, OK, USA). […]

Pipeline specifications

Software tools EthoVision, Statistica
Applications Miscellaneous, Macroscope & basic digital camera imaging
Organisms Rattus norvegicus
Diseases Substance-Related Disorders
Chemicals Amphetamine