Computational protocol: One library to make them all: Streamlining yeast library creation by a SWAp-Tag (SWAT) strategy

Similar protocols

Protocol publication

[…] Three algorithms were used to predict the presence of a signal peptide in each yeast protein, SignalP 4.1 , Phobius 1.01 , and Philius . We considered a protein as SP bearing for this study if all three programs predicted a SP in it, or if at least two programs predicted a SP with the exact same length ( and ). […]

Pipeline specifications

Software tools SignalP, Phobius, Philius
Application Protein sequence analysis
Organisms Saccharomyces cerevisiae