Dataset features


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Feb 5 2018
Last update date: Aug 23 2018
Access: Public
Diseases: Leukemia, Leukemia, Hairy Cell, Philadelphia Chromosome, Genetic Predisposition to Disease, Precursor Cell Lymphoblastic Leukemia-Lymphoma
Chemicals: Tyrosine
Dataset link pre-B cells from normal control, preleukemic, fully leukemic and fully leukemic, nilotinib-treated P190 BCR/ABL transgenic mice

Experimental Protocol

Mice transgenic for the human P190 form of Bcr/Abl (Jackson Labs strain 017833) develop precursor B-lineage (pre-B) acute lymphoblastic leukemia, on average within 3 months of birth, when on a C57Bl/6J background. Bone marrows were isolated from control C57BLl/6J mice and from transgenic Bcr/Abl mice when they had not yet developed full-blown leukemia (90 days of age), and from fully leukemic mice that had received a seven-day treatment with 75 mg/kg AMN107 (nilotinib). Three mice were used per condition, and cells from each mouse were processed separately. Pre-B cells from these twelve bone marrows were flow-sorted using CD19 and AA4.1 as markers. Total RNA from CD19+ AA4.1+cells used for microarray analysis was isolated by RNeasy (QIAGEN) purification. Double-strand complementary DNA was generated from 5 µg of total RNA using a poly(dT) oligonucleotide that contains a T7 RNA polymerase initiation site and the SuperScript III reverse transcription (Invitrogen). Biotinylated cRNA was generated and fragmented according to the Affymetrix protocol and hybridized to 430 mouse microarrays (Affymetrix).








Nora Heisterkamp