Computational protocol: The vacuolar-sorting protein Snf7 is required for export of virulence determinants in members of the Cryptococcus neoformans complex.

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Protocol publication

[…] The C. neoformans putative SNF7 gene sequence (Broad Institute accession number CNAG_01583.2) was identified by a BLAST search in the C. neoformans var. grubii strain H99 genomic database at the Broad Institute database by using the SNF7 sequence of C. gattii (Broad Institute accession number CNBG_3856). The putative amino acid sequences of Snf7 functional homologs in Saccharomyces cerevisiae (NCBI accession number P39929.1), Candida albicans (NCBI accession number EEQ43071.1), Aspergillus nidulans (Broad Institute accession number ANID_04240), Paracoccidioides brasiliensis (Broad Institute accession number PAAG_07890.1), Schizosaccharomyces pombe (NCBI accession number Q9P7F7), Neurospora crassa (Broad Institute accession number NCU01282.5), Magnaporthe oryzae (NCBI accession number EHA47375.1), Arabidopsis thaliana (NCBI accession number AEE85595.1), Oryza sativa (NCBI accession number AAT85290.1), C. neoformans (Broad Institute accession number CNAG_01583.2) and C. gattii (Broad Institute accession number CNBG_3856) were aligned using Clustal X2. Phylogenetic analysis was performed using Mega5 applying the neighbor-joining method. Tree architecture was inferred from 1,000 bootstraps. […]

Pipeline specifications

Software tools Clustal W, MEGA
Application Phylogenetics
Organisms Mus musculus, Cryptococcus neoformans, Saccharomyces cerevisiae, Homo sapiens, Cryptococcus gattii VGIII
Diseases Infection