Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 1
Release date: May 2 2008
Last update date: Mar 19 2012
Access: Public
Chemicals: Carbon, Iron, Sulfur
Dataset link Transcriptional Response of 0.1%YE-grown M. sedula cells to ferrous sulfate

Experimental Protocol


Dye flip of Mse cells includes two samples, yeast exact (YE) and yeast extract + ferrous sulfate (YEF). The first slide, Y3F5, has YE RNA labeled with cy3 and YEF RNA labeled with cy5, while the second slide, F3Y5, has YEF RNA labeled with cy3 and YE RNA labeled with cy5. YE serves as the reference condition, with the expectation that ORFs involved with Fe2+ oxidation (and SO4 metabolism) will be upregulated on YEF (log2 fold change of YE-YEF < -1).

Repositories


GEO

GSE11296

ArrayExpress

E-GEOD-11296

BioProject

PRJNA106717

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Contact


Kathryne Auernik
Kathryne Sherlock Auernik