Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Dec 31 2011
Last update date: Apr 18 2017
Access: Public
Genes: Hdac1, ZFEND, HDAC1, HDAC2, KDM5B, TP53
Taxon: Mus musculus
Dataset link Expression data from HDAC1 and HDAC2 knocked-out mouse oocyte

Experimental Protocol


Total RNA was extracted from 80 oocytes isolated from mice 12 days-of-age using the PicoPure RNA kit (Arcturus), amplified with the Ovation Pico WTA system (NuGen), and then fragmented and labeled with the FL-OvationTM cDNA Biotin Module V2 (NuGen). Four independent biological replicates were hybridized to Affymetrix GeneChip Mouse 1.1 ST microarrays (http://www.affymetrix.com/). Raw microarray data were analyzed as previously described using MAS5, GeneSpring v7, SAM and EASE software (56). A 1.4-fold cutoff was used for EASE analysis; four biological replicates provide sufficient statistical power and confidence to detect a 1.4-fold change in transcript abundance

Repositories


GEO

GSE34786

ArrayExpress

E-GEOD-34786

BioProject

PRJNA151059

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Contact


Hua Pan