Computational protocol: The elevation of plasma concentrations of apoB 48 containing lipoproteins in familial hypercholesterolemia is independent of PCSK9 levels

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Protocol publication

[…] Total plasma apoB concentrations were determined by a commercial enzyme-linked immunosorbent assay (ELISA) kit using immuno-purified polyclonal antibodies (Alerchek Inc., Portland, Maine, USA). Plasma apoB-48 levels were determined using a commercial ELISA kit using immuno-purified monoclonal antibodies (Shibayagi Co., Shibukawa, Gunma Prefecture, Japan) and no cross-reactivity with apoB-100 (<0.001%) has been reported []. Plasma PCSK9 concentrations were measured using a commercial ELISA kit (Circulex, CycLex, Nagano, Japan). [...] Plasma lipid and PCSK9 concentrations of the controls, HeFH and HoFH were compared using ANOVA models with the Tukey’s adjustment for multiple comparisons. Multiple linear regression model was used to assess independent associations between multiple variables and apoB-48 concentrations. Normality of the models was evaluated by the distribution of the scaled residual values. Spearman’s rank correlation test was used to evaluate the association between plasma apoB-48 and PCSK9 levels in controls and HeFH. All analyses were performed using JMP Pro software (v12.2.0, SAS Institute, Cary, NC). Statistical significance was considered at P < 0.05. […]

Pipeline specifications

Software tools cycleX, JMP Pro
Applications Miscellaneous, scRNA-seq analysis
Organisms Homo sapiens