Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 1
Release date: Apr 20 2015
Last update date: Apr 21 2015
Access: Public
Taxon: Cryptococcus neoformans, Neurospora crassa, Saccharomyces cerevisiae, Tremella mesenterica, Ustilago maydis
Dataset link Optimal codons in Tremella fuciformis end in C/G, a strong difference with known Tremella species

Experimental Protocol


Using the Total RNA was extracted from yeast-like cells of T. fuciformis Tr21 in their exponential growth stage, and double-stranded cDNA was synthesized for tag library construction and digital gene expression tag (DGE) sequencing at BGI Tech Solutions Co., Ltd (Shenzhen, China). Image analysis, base calling, extraction of tags, and tag counting were conducted using the Illumina pipeline. Clean tags were mapped to predicted gene models of the draft genome with a mismatch tolerance of 1bp. The number of tags for each CDS was calculated and then normalized to TPM (number of transcripts per million clean tags) digital gene expression (DGE)

Repositories


GEO

GSE68023

ArrayExpress

E-GEOD-68023

ENA

SRP057416

BioProject

PRJNA281545

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Contact


Baogui Xie