Computational protocol: The Optimized Fabrication of Nanobubbles as Ultrasound Contrast Agents for Tumor Imaging

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Protocol publication

[…] The cancer xenograft model was established by the subcutaneous injection of 5 × 106 MDA-MB-231 tumor cells into the right side dorsal scapular area of female athymic nude mice. Approximately 2 to 4 weeks after inoculation, based on the caliper measurements of perpendicular dimensions, the tumor volume was determined to be approximately 700 to 1000 mm3. All animal experiments were performed according to the guidelines issued by the local committee on Animal Care and Use, which are in accordance with the National Institutes of Health (NIH) guidelines. The ultrasound-enhanced tumor images were recorded using an Esaote MyLab Twice ultrasound unit with a broad bandwidth (3–9 MHz) routine clinical linear array transducer (Esaote, LA522). All images and films were recorded as digital files for subsequent playback and image analysis. Nanobubbles (150 μl) were injected into each mouse under isoflurane anesthesia through the tail vein, and the enhanced tumor and liver images were recorded immediately after injection until the end of the enhancement. After the nanobubble imaging, the nanobubbles were allowed to clear the circulation system of the mice for 2 h. Subsequently, the enhanced tumor images were recorded for SonoVue using the same volume and imaging protocol. Images were collected at 10 s, 30 s, 1 min, 2 min and 5 min post-injection for both nanobubbles and SonoVue. All digital clips and images were stored for gray-scale analysis, which was performed using WCIF ImageJ software (v1.37; National Institutes of Health, Bethesda, MA). Time-intensity curves for the nanobubble and SonoVue images were created and analyzed statistically using SPSS software (v19.0; SPSS Inc., an IBM Company). […]

Pipeline specifications

Software tools ImageJ, SPSS
Applications Miscellaneous, Microscopic phenotype analysis
Diseases Neoplasms, Drug-Related Side Effects and Adverse Reactions