|Application:||Gene expression microarray analysis|
|Number of samples:||30|
|Release date:||Jul 6 2010|
|Last update date:||Mar 22 2012|
|Chemicals:||Erythromycin, Sulfamethazine, Sulfamethoxazole|
|Dataset link||Meta-transcriptomic analysis of the response of river biofilms to pharmaceutical products using anonymous DNA microarray|
The reactor design for biofilm development has been previously described (Lawrence et al., 2004; Lawrence et al., 2000). Two duplicate experiments were carried out, with reactors being inoculated with either water from the WC (nutrient rich) or the SSR (nutrient poor). Treatments consisted in the addition of various pharmaceutical compounds: 1 µg l-1 erythromycin (ER), 1 µg l-1 gemfibrozil (GM), 0.5 µg l-1 sulfamethazine (SN), 0.5 µg l-1 sulfamethoxazole (SL). Nothing was added to control reactors (CO). All treatments were replicated independently three times. A reference sample (composite sample from Wascana Creek reactors used to construct the microarray) was hybridized (Cy5) on each slide.