Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 30
Release date: Jul 6 2010
Last update date: Mar 22 2012
Access: Public
Chemicals: Erythromycin, Sulfamethazine, Sulfamethoxazole
Dataset link Meta-transcriptomic analysis of the response of river biofilms to pharmaceutical products using anonymous DNA microarray

Experimental Protocol


The reactor design for biofilm development has been previously described (Lawrence et al., 2004; Lawrence et al., 2000). Two duplicate experiments were carried out, with reactors being inoculated with either water from the WC (nutrient rich) or the SSR (nutrient poor). Treatments consisted in the addition of various pharmaceutical compounds: 1 µg l-1 erythromycin (ER), 1 µg l-1 gemfibrozil (GM), 0.5 µg l-1 sulfamethazine (SN), 0.5 µg l-1 sulfamethoxazole (SL). Nothing was added to control reactors (CO). All treatments were replicated independently three times. A reference sample (composite sample from Wascana Creek reactors used to construct the microarray) was hybridized (Cy5) on each slide.

Repositories


GEO

GSE20501

ArrayExpress

E-GEOD-20501

BioProject

PRJNA125083

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Contact


Etienne Yergeau