Dataset features

Specifications


Application: Gene expression microarray analysis, miRNA array analysis
Number of samples: 6
Release date: Oct 27 2009
Last update date: Jun 26 2012
Access: Public
Genes: CD34
Dataset link Absolute quantification of miRNAs

Experimental Protocol


Total liver RNA was mixed with 2.5 fmol of each of 18 RNA oligonucleotides reverse complement to miRControl 3 probes and subsequently fluorescently labelled by 3’ ligation. Total RNA mix was hybridized in a dual colour approach to microarrays versus a second labelled synthetic miRNA pool (n = 6). The synthetic miRNA pool consisted of 2.5 fmol of each of 891 non redundant miRNAs sequences and miRControl 3 sequences. The array data was normalized by calculating the median of the miRControl 3 present in the liver and UR sample. The miRNA amount was calculated with respect to the corresponding miRNA in the UR.

Repositories


GEO

GSE15835

ArrayExpress

E-GEOD-15835

BioProject

PRJNA116765

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Contact


Ute Bissels