Computational protocol: Apolipophorin-III Mediates Antiplasmodial Epithelial Responses in Anopheles gambiae (G3) Mosquitoes

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Protocol publication

[…] The Spodoptera litura ApoLp-III cDNA sequence was chosen to identify a putative AgApoLp-III cDNA from the Ag genome database using the NCBI basic local alignment search tool (BLAST). Putative AgApoLp-III EST sequences were identified and used to design specific primers to amplify, clone, and sequence the cDNA. The sequence of the cloned AgApoLp-III cDNA () was used to further investigate the genomic structure of AgApoLp-III by using the BLASTN program. Sequences of apoLp-IIIs from other species were obtained from NCBI, aligned, and an amino acid (aa) identity table built using Clustal-X2 (version 2.0.11) software . Phylogenetic analysis was conducted using the neighbor-joining method with bootstrap test (1,000 replicates) using the MEGA program (version 4.0.2) . […]

Pipeline specifications

Software tools BLASTN, Clustal W, MEGA
Application Phylogenetics
Organisms Plasmodium berghei, Anopheles gambiae, Drosophila melanogaster, Manduca sexta