Dataset features


Application: Gene expression microarray analysis
Number of samples: 19
Release date: Jul 15 2010
Last update date: Jul 1 2016
Access: Public
Diseases: Infection, Neoplasms, Bone Tissue
Dataset link Gene expression data from P. gingivalis infected Mouse

Experimental Protocol

P. gingivalis was injected at 1.5 x 10E9 (N = 10) into the soft tissues overlying the calvaria of the mice for 3 days. A control group (N = 9) was injected with vehicle once daily for 3 days. Mice were euthanized 8 h after the last injection. The calvarial bones and overlying soft tissues from 5 mice in each group were excised, snap frozen in liquid nitrogen, and stored at –80°C until RNA isolation. Total RNA was isolated from the frozen calvarial tissue and calvarial bone from each mouse (P. gingivalis infected and control animals) with Trizol reagent (Invitrogen, CA). Equal amounts of RNA from samples were labeled and hybridized on a mouse GeneChip following the protocol described in the GeneChip Expression Analysis Technical manual (Affymetrix, Santa Clara, CA). After hybridization, the GeneChip arrays were stained and scanned in an Affymetrix GCS 3000 7G Scanner.