|Number of samples:||135|
|Release date:||Nov 19 2018|
|Last update date:||Nov 26 2018|
|Dataset link||Bulk RNA sequencing of chemical-induced intermediate cells|
RNA-seq was modified from a previous method. Briefly, RNA was extracted from samples by Trizol reagent (TAKARA), reverse transcription was conducted by SuperScript II reverse transcriptase (Invitrogen), double strand cDNA was conducted using NEBNext mRNA second strand synthesis kit (NEB), double strand DNA was cleaned with AMPure XP beads (Beckman Coulter). 3’end enriched sequencing library was constructed with Nextera XT kit (Illumina) and sequenced on Illumina X-Ten platform. There were 3 samples for each group and 5 replicates of sequencing library for each sample.