Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 135
Release date: Nov 19 2018
Last update date: Nov 26 2018
Access: Public
Genes: ELF4
Taxon: Mus musculus
Dataset link Bulk RNA sequencing of chemical-induced intermediate cells

Experimental Protocol


RNA-seq was modified from a previous method. Briefly, RNA was extracted from samples by Trizol reagent (TAKARA), reverse transcription was conducted by SuperScript II reverse transcriptase (Invitrogen), double strand cDNA was conducted using NEBNext mRNA second strand synthesis kit (NEB), double strand DNA was cleaned with AMPure XP beads (Beckman Coulter). 3’end enriched sequencing library was constructed with Nextera XT kit (Illumina) and sequenced on Illumina X-Ten platform. There were 3 samples for each group and 5 replicates of sequencing library for each sample.

Repositories


GEO

GSE118235

ENA

SRP156769

BioProject

PRJNA485016

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Contact


Yishan Chen