Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 40
Release date: Sep 21 2018
Last update date: Nov 27 2018
Access: Public
Genes: ITGAM, CDK1, KRT20, ITGAX, CD14, CD1C, CD4, NCAM1, ISG20, PTPRC
Dataset link Transcriptional profile of human neonatal cord blood and adult peripheral blood dendritic cells

Experimental Protocol


Dendritic cell subsets were isolated from 3 peripheral blood donors and 3 cord blood donors. Monocytes were isolated from 3 cord blood donors. Neonatal naive T cells were isolated from 1 peripheral blood donor. Dendritic cells were left unstimulated or were stimulated with either poly I:C + R848, Bacillus Calmette–Guérin (BCG) or Respiratory Syncytial Virus (RSV) for 6h. RNA from 5000 cells was amplified, labeled, and hybridized to an an Agilent Whole Human Genome Oligo Microarrays 8x60K.

Repositories


GEO

GSE104110

BioProject

PRJNA408282

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Contact


Sebastien LEMOINE
Xiaoming Zhang