Computational protocol: Crystal structures of human BTG2 and mouse TIS21 involved in suppression of CAF1 deadenylase activity

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Protocol publication

[…] For structure determination of human BTG2, the phasing problem was solved by the MR method using the program Phaser in the CCP4 program suite (). The structure of TOB from the TOB-CAF1 complex (PDB entry 2D5R) (), which shares 40% sequence identity with human BTG2, was employed as a search model. The human BTG2 model was manually adjusted with COOT and refined with Refmac5 (,). The final model is composed of one BTG2 monomer, with 120 residues (from residues 7 to 127) and 55 water molecules. The structure of mouse TIS21 was also solved by MR following the same protocol and using the same search model. The final TIS21 model includes 117 residues (from residues 8 to 125) and 82 water molecules. Data collection and structure refinement statistics are summarized in . […]

Pipeline specifications

Software tools CCP4, Coot, REFMAC5
Application Protein structure analysis
Organisms Mus musculus, Homo sapiens