Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 60
Release date: Jun 23 2016
Last update date: Oct 26 2018
Access: Public
Diseases: Neuroblastoma, Radial Neuropathy
Dataset link A Primate lncRNA Mediates Notch Signaling During Neuronal Development by Sequestering miRNA [single cell sequencing analysis]

Experimental Protocol


Cerebral organoids were generated as in LancasterĀ et al. (Lancaster and Knoblich, 2014). Organoids were dissociated into single cells and captured on C1 Single-Cell Auto Prep Integrated Fluidic Circuit (IFC) (Fluidigm). The RNA extraction and amplification was performed on the chip as described by the manufacturer. We captured 68 single-cells on a C1 Single-Cell Auto Prep System (Fluidigm) and sequenced the RNA on a NextSeq500 System (Illumina) (Pollen et al., 2014). Out of 68 cells, we obtained 60 high quality cells.

Repositories


GEO

GSE74207

ArrayExpress

E-GEOD-74207

ENA

SRP065040

BioProject

PRJNA299330

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