Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 9
Release date: Feb 8 2013
Last update date: Aug 23 2018
Access: Public
Diseases: Neoplasms
Chemicals: Sirolimus
Dataset link Gene expression data from rapamycin resistant and sensitive cell lines

Experimental Protocol


Total RNA extraction and hybridization on Affymetrix microarrays of rapamycin sensitive (RS) cells (BC3H1, mouse brain tumor cell line with myogenic properties, ATCC) cultured in Dulbecco’s modified essential medium (DMEM) media supplemented with 20% fetal bovine serum (FBS), penicillin (100 U/ml) and streptomycin (100 mg/ml). Rapamycin resistant cells (RR1) were developed by culturing BC3H1 cells in the presence of 1 uM rapamycin for 6 months. Three samples in triplicates: 1) Rapamycin sensitive cells treated with DMSO for 24 h(BC3H1, reference), 2) Rapamycin sensitive cells treated for 24 h with 100 nM rapamycin (BC3H1+R), 3) Rapamycin resistant cells constantly treated with 1uM Rapamycion (RR1+R).

Repositories


GEO

GSE19885

ArrayExpress

E-GEOD-19885

BioProject

PRJNA123699

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