Dataset features

Specifications


Dataset type: Non-coding RNA profiling by array
Number of samples: 12
Release date: Dec 23 2018
Last update date: Jan 16 2019
Access: Public
Dataset link MicroRNA expression profile of HCT-8 in the early phase of Cryptosporidium parvum infection

Experimental Protocol


In the study presented here,the miRNAs expression profiles of HCT-8 cells at 4 and 12 h post-Cryptosporidium parvum infection were analyzed via microarray, qPCR, and bioinformatic analyses. Infected and uninfected HCT-8 cells were collected at 4h and at 12h. All HCT-8 cells were washed three times with PBS, and 1 ml TRIZOL reagent (Invitrogen, Carlsbad, CA, USA) was added to each well. Total RNA was isolated according to the manufacturer’s protocols.All assays were performed in triplicate.

Repositories


GEO

GSE95365

BioProject

PRJNA376743

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