|Dataset type:||Non-coding RNA profiling by array|
|Number of samples:||12|
|Release date:||Dec 23 2018|
|Last update date:||Jan 16 2019|
|Dataset link||MicroRNA expression profile of HCT-8 in the early phase of Cryptosporidium parvum infection|
In the study presented here,the miRNAs expression profiles of HCT-8 cells at 4 and 12 h post-Cryptosporidium parvum infection were analyzed via microarray, qPCR, and bioinformatic analyses. Infected and uninfected HCT-8 cells were collected at 4h and at 12h. All HCT-8 cells were washed three times with PBS, and 1 ml TRIZOL reagent (Invitrogen, Carlsbad, CA, USA) was added to each well. Total RNA was isolated according to the manufacturer’s protocols.All assays were performed in triplicate.