|Application:||Gene expression microarray analysis|
|Number of samples:||9|
|Release date:||Apr 20 2006|
|Last update date:||Mar 16 2012|
|Computational protocol:||TM4, Spotfinder, PSORTb, Clustal W, Comprehensive Microbial Resource, MetaCyc|
|Dataset link||Transcript profiling in A. pleuropneumoniae under iron restriction|
The bacteria was grown in BHI broth with or without 50 ug/ml EDDHA to generate iron restriction. RNA extractions were performed on five different days using the QIAGEN RNeasy Kit, and a total of nine hybridization were performed, including three pairs of dye swap experiments and a pair of hybridizations being a direct repeat (same dyes). Data were acquired using a Perkin-Elmer ScanArray Express scanner, and were quantified, normalized and analysed using the SpotFinder, MIDAS and TMEV software from TIGR's TM4 Suite of Microarray Analysis softwares.
DOI: 10.1186/1471-2164-8-72call_split See protocol