Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 9
Release date: Apr 20 2006
Last update date: Mar 16 2012
Access: Public
Diseases: Pleuropneumonia
Chemicals: Iron
Computational protocol: TM4, Spotfinder, PSORTb, Clustal W, Comprehensive Microbial Resource, MetaCyc
Dataset link Transcript profiling in A. pleuropneumoniae under iron restriction

Experimental Protocol


The bacteria was grown in BHI broth with or without 50 ug/ml EDDHA to generate iron restriction. RNA extractions were performed on five different days using the QIAGEN RNeasy Kit, and a total of nine hybridization were performed, including three pairs of dye swap experiments and a pair of hybridizations being a direct repeat (same dyes). Data were acquired using a Perkin-Elmer ScanArray Express scanner, and were quantified, normalized and analysed using the SpotFinder, MIDAS and TMEV software from TIGR's TM4 Suite of Microarray Analysis softwares.

Repositories


GEO

GSE4674

BioProject

PRJNA96775

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Contact


Vincent Deslandes

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