Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Sep 9 2010
Last update date: Oct 29 2018
Access: Public
Diseases: Infection
Dataset link A cryptic sensor for HIV-1 activates antiviral innate immunity in dendritic cells

Experimental Protocol


We analyzed the gene expression profiles of uninfected human monocyte-derived dendritic cells (MDDCs) and MDDCs infected with an envelope-defective GFP-encoding VSV-G-pseudotyped HIV-1 vector (HIVGFP(G)) and with VSV-G pseudotyped virus-like particles derived from SIVmac to deliver Vpx (SIVVLP(G)), alone or in combination. Cells were infected at day 4 of differentiation and cells were harvested 48 hours later. RNA was extracted with TRIzol. RNA was labeled and hybridized to Human Genome U133A 2.0 arrays arrays following the Affymetrix protocols. Data were analyzed in R and Bioconductor.

Repositories


GEO

GSE22589

ArrayExpress

E-GEOD-22589

BioProject

PRJNA128389

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Contact


Dan Littman

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