Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 4
Release date: Jul 11 2013
Last update date: Nov 3 2017
Access: Public
Taxon: Caenorhabditis elegans
Dataset link Snyder_FKH-10_GFP_L4

Experimental Protocol


EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: OP337(official name : OP337 genotype : unc119(ed3);wgIs377(fkh-10::TY1 EGFP FLAG;unc119) outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The FKH-10::EGFP fusion protein is expressed in the correct fkh-10 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the FKH-10 transcription factor. made_by : Unknown ); Developmental Stage: L4; Genotype: unc119(ed3);wgIs377(fkh-10::TY1 EGFP FLAG;unc119); Sex: Hermaphrodite; EXPERIMENTAL FACTORS: Developmental Stage L4; Target gene fkh-10; Strain OP337(official name : OP337 genotype : unc119(ed3);wgIs377(fkh-10::TY1 EGFP FLAG;unc119) outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The FKH-10::EGFP fusion protein is expressed in the correct fkh-10 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the FKH-10 transcription factor. made_by : Unknown ); temp (temperature) 20 degree celsius

Repositories


GEO

GSE48737

ArrayExpress

E-GEOD-48737

ENA

SRP028466

Download


Contact


DCC modENCODE