Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Apr 1 2015
Last update date: May 15 2017
Access: Public
Taxon: Homo sapiens
Dataset link Translatome and transcriptome profiling of SH-SY5Y cells treated with staphylococcal alpha-hemolysin oligomers

Experimental Protocol


The comparison between translatome and transcriptome profiling was used to discover mRNA-specific changes of the SH-SY5Y cells transcriptome and translatome in response to staphylococcal α-hemolysin oligomers (rAHL). To identify translationally regulated mRNAs, gene expression signals derived from the polysomal mRNA populations were compared by microarrays analysis to those obtained from total RNAs. Polysomal mRNA and total mRNA were isolated from SH-SY5Y cells treated with 3nM of extracted oligomers (rAHL) for 2 hours. Cells lysates were collected from untreated cells (control) and from treated cells. All experiments were run in biological triplicates.

Repositories


GEO

GSE60543

ArrayExpress

E-GEOD-60543

BioProject

PRJNA258509

Download


Contact


Toma Tebaldi