|Application:||Gene expression microarray analysis, miRNA array analysis|
|Number of samples:||8|
|Release date:||Oct 21 2011|
|Last update date:||Feb 6 2015|
|Diseases:||Auditory Diseases, Central, Neoplasms, Leukemia, Myelogenous, Chronic, BCR-ABL Positive|
|Dataset link||Distinctive microRNA expression profile and potential funtional role in K562 cells|
MiRNA profiling of CML: With the objective of deciphering a potential miRNA expression signature associated with CML, we analyzed the miRNAs expression profile of K562 in reference to a pool of 3 healthy blood samples using an miRNA-based microarray chip assay. In addition, to search for Bcr-Abl-dependent or Src-dependant miRNA expression patterns, K562 cells were treated or not with imatinib or with dasatinib to inhibit Bcr-Abl tyrosine kinase activity or Bcr-Abl and Src tyrosine kinase activity, respectively. In order to validate the microarray data and to ensure that the variability observed among samples was not technical, we carried out Taqman miRNA quantitative real-time PCR analysis (Applied Biosystems, USA) on miRNAs that we find to be most relevant, according to bioinformatics analysis (see below) and published data.
Citations per year