Dataset features

Specifications


Application: Gene expression microarray analysis, miRNA array analysis
Number of samples: 8
Release date: Oct 21 2011
Last update date: Feb 6 2015
Access: Public
Diseases: Auditory Diseases, Central, Neoplasms, Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Dataset link Distinctive microRNA expression profile and potential funtional role in K562 cells

Experimental Protocol


MiRNA profiling of CML: With the objective of deciphering a potential miRNA expression signature associated with CML, we analyzed the miRNAs expression profile of K562 in reference to a pool of 3 healthy blood samples using an miRNA-based microarray chip assay. In addition, to search for Bcr-Abl-dependent or Src-dependant miRNA expression patterns, K562 cells were treated or not with imatinib or with dasatinib to inhibit Bcr-Abl tyrosine kinase activity or Bcr-Abl and Src tyrosine kinase activity, respectively. In order to validate the microarray data and to ensure that the variability observed among samples was not technical, we carried out Taqman miRNA quantitative real-time PCR analysis (Applied Biosystems, USA) on miRNAs that we find to be most relevant, according to bioinformatics analysis (see below) and published data.

Repositories


GEO

GSE28825

ArrayExpress

E-GEOD-28825

BioProject

PRJNA138785

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Contact


Metsada Pasmanik-Chor

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