Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 6
Release date: Feb 16 2017
Last update date: Nov 28 2018
Access: Public
Diseases: Neoplasms
Dataset link Transcriptome of iPSC-derived Cerebral Organoids with Heterozygous Knockout in CHD8

Experimental Protocol


iPSCs derived from a healthy subject were transduced with CRISPR/Cas9 vectors with single guide RNA sequences to target the N-terminal of CHD8 protein to generate truncated mutations. The CHD8+/- iPSC lines were used to generate cerebral organoids for RNA-seq analysis, together with samples prepared from the parental clones, for a total of 6 samples (two biological replicates of wild-type (WT) and 4 biological replicates of CHD8+/-).

Repositories


GEO

GSE85417

ENA

SRP081211

BioProject

PRJNA338401

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Contact


Deyou Zheng

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