Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Mar 12 2013
Last update date: Jun 13 2013
Access: Public
Chemicals: Methicillin, Vancomycin
Dataset link Comparison of the transcriptional profiles of intermediately vancomycin resistant and susceptible S. aureus strains

Experimental Protocol


For transcriptional profiling, the strains compared were grown in BHI to an optical density (600 nm) of 0.8-1.0. To guarantee statistical significance of the data, each experiment was replicated 4 times including a dye swap. RNA was isolated from two independent cultures of each strain. Each RNA preparation was used twice to synthesize cDNA resulting in four chips per competitive comparison.

Repositories


GEO

GSE10529

BioProject

PRJNA107881

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Contact


Peter Sass

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