|Number of samples:||12|
|Release date:||Jan 24 2017|
|Last update date:||Feb 26 2018|
|Dataset link||Mercury-Induced Epigenetic Transgenerational Inheritance of Abnormal Neurobehavior is Correlated with Sperm Epimutations in Zebrafish|
F1 generation zebrafish embryos were exposed to MeHg. F1 generation control and MeHg-exposed lineages propogated to the F3 generation. Sperm was collected from both F1 and F3 generation fish. The sperm from two fish was combined to create a single pooled sample. Three pools were collected for both control and MeHg treated groups. The methylation characteristics of the pooled samples was estimated using a methylated DNA immunoprecipitation (MeDIP) followed by PE50 sequencing on an Illumina HiSeq 2500 platform.