Dataset features

Specifications


Application: BS-seq analysis
Number of samples: 12
Release date: Jan 24 2017
Last update date: Feb 26 2018
Access: Public
Chemicals: Mercury
Dataset link Mercury-Induced Epigenetic Transgenerational Inheritance of Abnormal Neurobehavior is Correlated with Sperm Epimutations in Zebrafish

Experimental Protocol


F1 generation zebrafish embryos were exposed to MeHg. F1 generation control and MeHg-exposed lineages propogated to the F3 generation. Sperm was collected from both F1 and F3 generation fish. The sperm from two fish was combined to create a single pooled sample. Three pools were collected for both control and MeHg treated groups. The methylation characteristics of the pooled samples was estimated using a methylated DNA immunoprecipitation (MeDIP) followed by PE50 sequencing on an Illumina HiSeq 2500 platform.

Repositories


GEO

GSE89144

ENA

SRP092038

BioProject

PRJNA350395

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Contact


Michael Skinner