Computational protocol: The effects of graded levels of calorie restriction: IX. Global metabolomic screen reveals modulation of carnitines, sphingolipids and bile acids in the liver of C57BL/6 mice

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Protocol publication

[…] Information on experimental set‐up can be found in Data S11.Single intraperitoneal S1P (100 ng and 200 ng), SEW2871 (100 ng and 200 ng) or saline control injections (triplicates) were performed at 16 weeks. Injections were performed at 1000 h, after weighing. S1P and SEW2871 were obtained from the Cayman Chemical Company (Ann Arbor, MI, USA). Dosages were based on Silva et al. () and Dong et al. (). For analysis of Tb, data were averaged between 1230 h and 1330 h (n = 5). This time frame was chosen as Tb had stabilized after increasing from handling and injection, but was 7 h before lights out when activity and Tb increase due to diurnal rhythms. Food intake (g) was split into light (11:00–16:00) and dark phases (16:00–04:00) and summed across mice (n = 6). To determine whether high and low S1P and SEW2871 had an effect on Tb and food intake, we used a linear mixed‐effects modelling approach using the nlme package (Pinheiro et al., ). Injection treatment (saline control, S1P high, S1P low, SEW2871 high and SEW2871 low) and activity level were used as fixed effects and mouse ID was used as a random effect. Model selection was performed by minimizing the Akaike information criterion (AIC) and model validation performed using standard residual plots (Bozdogan, ).All statistical analyses were performed using the R statistical environment (R Core Team, ). […]

Pipeline specifications

Software tools lme4, nlme
Application Mathematical modeling
Organisms Mus musculus
Chemicals Bile Acids and Salts, Carnitine, Fatty Acids, Methionine, Sphingolipids, Taurocholic Acid