Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 24
Release date: Dec 31 2017
Last update date: Dec 31 2017
Access: Public
Taxon: Cicer arietinum
Dataset link Transcriptome dynamics during seed development in chickpea (Himchana)

Experimental Protocol


We collected leaves from the field-grown chickpea plants during mid maturation stage of seed development. Seven different stages of seeds (whole seed) representing the major events occurring within the seed, were collected from the field-grown plants. The tissues were collected in at least three biological replicates. Total RNA isolated from these tissue samples was subjected to Illumina sequencing. The sequenced data was further filtered using NGS QC Toolkit to obtain high-quality reads. The filtered reads were mapped to annotated chickpea genome using TopHat and fragments per exon kilobase per million (FPKM) was calculated using Cufflinks software for each gene in all the samples to measure their gene expression. Differential expression analysis was performed using Cuffdiff software. The differentially expressed genes during various stages of seed development were identified.

Repositories


GEO

GSE79720

ENA

SRP072564

BioProject

PRJNA316844

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