Computational protocol: Progressive mitochondrial protein lysine acetylation and heart failure in a model of Friedreich’s ataxia cardiomyopathy

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Protocol publication

[…] Ventricular tissue selected for histology was fixed in 10% formalin and paraffin embedded. Histological analysis included hematoxylin and eosin (H&E) and Masson’s Trichrome to detect collagen as a measure of fibrosis. Collagen quantification was performed using ImageJ (IJ1.46) to measure percent area of tissue positively stained for collagen. Three to five 20x digital micrographs were obtained from each tissue section that underwent quantification studies. [...] All calculations, analyses and graphs were performed using SigmaPlot (Systat Software, Inc.). Two groups were compared using a two-tailed student’s t test for samples with equal variance. Final data are presented as mean (±SD). Alpha was set at 0.05 and power at 0.800. A p-value of <0.05 was considered statistically significant. […]

Pipeline specifications

Software tools ImageJ, SigmaPlot
Applications Miscellaneous, cryo-EM, Microscopic phenotype analysis
Organisms Mus musculus