Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: May 25 2010
Last update date: Jan 27 2016
Access: Public
Diseases: Breast Neoplasms, Neoplasms, Inflammatory Breast Neoplasms
Dataset link Gene expression profiling of MCF7 breast cancer xenografts overexpressing podoplanin (human array)

Experimental Protocol


MCF7 human breast carcinoma cells were stably transfected with the pTet-Off-regulator plasmid and the pTRE2hyg response plasmid, comprising or not full-length cDNA of human podoplanin (transcript variant 1, GenBank Accession number NM_006474). Three podoplanin-overexpressing and three control clones were isolated and each of them was injected bilaterally into the mammary fat pads of five female nude mice. Tumors were harvested after 24 days of growth and RNA was extracted from each of them. RNA of the 10 tumors derived from the same cell clone was pooled to give a total of six samples for microarray analysis (3x podoplanin-overexpressing, 3x control).

Repositories


GEO

GSE14550

BioProject

PRJNA114509

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Contact


Leah Cueni