|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Sep 16 2011|
|Last update date:||Oct 29 2018|
|Taxon:||Homo sapiens, Rattus norvegicus|
|Dataset link||Human Mesenchymal Stem Cells in Cardiomyocyte Co-Culture|
A myocardial microenvironment was generated by dissociating neonatal rat hearts and establishing cardiomyocyte primary cultures. HumanMSCs constitutively labeled with dsRed localized to the cell's mitochondria were either grown separately (control) or added to the cardiomyocyte primary cultures and grown for 4 days. dsRed fluorescent hMSCs were harvested from co-cultures at 4 days using a FACscan flow cytometer. The RNA for the microarray analysis was prepared from three biologically separate samples of hMSCs co-cultured for 4 days and from hMSCs grown separately for 4 days (control).
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