Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Sep 16 2011
Last update date: Oct 29 2018
Access: Public
Taxon: Homo sapiens, Rattus norvegicus
Dataset link Human Mesenchymal Stem Cells in Cardiomyocyte Co-Culture

Experimental Protocol


A myocardial microenvironment was generated by dissociating neonatal rat hearts and establishing cardiomyocyte primary cultures. HumanMSCs constitutively labeled with dsRed localized to the cell's mitochondria were either grown separately (control) or added to the cardiomyocyte primary cultures and grown for 4 days. dsRed fluorescent hMSCs were harvested from co-cultures at 4 days using a FACscan flow cytometer. The RNA for the microarray analysis was prepared from three biologically separate samples of hMSCs co-cultured for 4 days and from hMSCs grown separately for 4 days (control).

Repositories


GEO

GSE32171

ArrayExpress

E-GEOD-32171

BioProject

PRJNA147425

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Contact


Barbara Muller-Borer

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