Computational protocol: cDNA cloning, characterization and expression analysis of peroxiredoxin 5 gene in the ridgetail white prawn Exopalaemon carinicauda

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Protocol publication

[…] The nucleotide and deduced amino acid sequences of EcPrx5 cDNA were analyzed and compared using the BLAST search programs (http://www.blast.ncbi.nlm.nih.gov/Blast.cgi). The multiple sequence alignment of Prx5 amino acid sequences was performed using the programs of Vector NTI advance 10.3 (Invitrogen). A phylogenetic NJ tree of Prxs was constructed by the MEGA 4.0 software []. […]

Pipeline specifications

Software tools Vector NTI Advance, MEGA
Applications Phylogenetics, Protein sequence analysis
Organisms Palaemon carinicauda, Macrobrachium rosenbergii, Megachile rotundata, Harpegnathos saltator, Acromyrmex echinatior, Danio rerio, Homo sapiens, Vibrio anguillarum
Diseases Bacterial Infections, Dental Caries, Drug-Related Side Effects and Adverse Reactions
Chemicals Oxygen