Dataset features


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Sep 21 2007
Last update date: Mar 17 2012
Access: Public
Chemicals: Ethanol, Nicotine
Dataset link Gene expression in the human VTA of cases with alcohol abuse

Experimental Protocol

RNA was extracted individually from postmortem tissue samples of human ventral tegmental area (VTA) of 6 cases with and 6 cases without chronic alcohol abuse. RNA was also extracted from various other brain region (nucleus accumbens, prefronatl cortex). All samples were linearly amplified individually as described. Reference sample: Part of the aRNA from the VTA and those of the other brain regions was pooled. Cy3 dCTP labelling of each individual VTA aRNA sample. Cy5 dCTP labelling of reference pool. One labelled VTA aRNA sample was hybridised together with labelled reference aRNA. Samples of the same pathology (alcoholics or control cases) were treated as biological replicates. Student's t-test was used to reveal differentially expressed genes.








Traute Flatscher-Bader