Computational protocol: Overexpression of an isopentenyl diphosphate isomerase gene to enhance trans-polyisoprene production in Eucommia ulmoides Oliver

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Protocol publication

[…] The deduced amino acid sequence of EuIPI was aligned against those of other IPIs from different organisms, including plants, bacteria, fungi, and animals, by the Clustal W method with default parameters (Slow-Accurate) using Lasergene® MegAlign (DNASTAR, Madison, USA). A phylogenetic tree was constructed by using the neighbor-joining method [] using Treeview (Glasgow, Scotland, UK). […]

Pipeline specifications

Software tools Clustal W, TreeViewX
Application Phylogenetics
Organisms Escherichia coli, Hevea brasiliensis
Chemicals Terpenes