Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 2
Release date: Mar 22 2010
Last update date: Aug 28 2018
Access: Public
Dataset link Mouse primary megakaryocytes, wild type & NF-E2p45-deficient

Experimental Protocol


To obtain wild type and p45-null fetal livers, p45+/- mice were crossed. Whole livers were recovered from mouse fetuses at E14.5 and single cell suspensions were prepared by successive passage through 25-gauge needles. Fetal liver cells were maintained in RPMI1640 (Wako) supplemented with 20% charcoal-stripped fetal bovine serum, 50 units/ml penicillin, 50 µg/ml streptomycin, and 50 ng/ml of recombinant human thrombopoietin. CD41+ cells were selected from a 4 day primary culture of E14.5 fetal liver cells using a MACS magnetic system (Miltenyi Biotec). The fetal liver culture with TPO described above was incubated with FITC-conjugated anti-CD41 antibody (BD Pharmingen, clone MWReg30) followed by incubation with anti-FITC microbeads. Subsequently, the microbeads were selected magnetically through MACS large cell columns (Miltenyi Biotec). Total RNA was extracted from the CD41+ cells using Isogen (Nippon Gene).

Repositories


GEO

GSE15581

ArrayExpress

E-GEOD-15581

BioProject

PRJNA115765

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Contact


Hozumi Motohashi