Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 3
Release date: Jul 31 2009
Last update date: Nov 3 2017
Access: Public
Taxon: Caenorhabditis elegans
Dataset link Identification of transcription factor CEH-14 binding sites in C.elegans

Experimental Protocol


CEH-14 was tagged with a dual GFP:3xFLAG tag at the carboxy terminus, whose expression pattern was confirmed through both fluorescence imaging and immunoblot analysis. The binding sites were determined using ChIP-seq. Two biological replicates were used in this study, but replicate1 is at a later L2 stage. Only one Input sample (non-immunoprecipitated chromatin (input) from replicate2 was used as a control. The concordance between two replicates is just above 50% when p-value is 0.05.

Repositories


GEO

GSE17454

ArrayExpress

E-GEOD-17454

ENA

SRP001580

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