Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Aug 31 2007
Last update date: Jul 1 2016
Access: Public
Chemicals: Ethanol, Glucose
Dataset link Prolonged selection in aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae

Experimental Protocol


A crucial feature of bakers' yeast is its capacity to produce CO2, referred to as fermentative capacity (van Hoek et al., 1998). After prolonged glucose-limited cultivation of S. cerevisiae, in addition to an increased affinity for glucose, we observed a dramatic decrease in fermentative capacity. Consequently, the aim of the present study was to perform an integral analysis of the long-term adaptation of S. cerevisiae during prolonged glucose-limited, aerobic cultivation in chemostat cultures, with special emphasis on the regulation of glucose transport and glycolytic capacity. To this end, we applied an integrated approach that combined transcriptome analysis, measurement of fermentative capacity and activities of glucose transport and glycolytic enzymes, and characterization of cellular morphology.

Repositories


GEO

GSE8898

ArrayExpress

E-GEOD-8898

BioProject

PRJNA102289

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Contact


Jean-Marc Daran