Dataset features

Specifications


Dataset type: Genome binding/occupancy profiling by array, Epigenomics
Number of samples: 3
Release date: Jul 19 2018
Last update date: Oct 19 2018
Access: Public
Taxon: Homo sapiens
Dataset link Divergence in DNA specificity among paralogous transcription factors contributes to their differential in vivo binding [uPBM_E2f1E2f3E2f]

Experimental Protocol


Universal protein-binding microarray (PBM) experiments were performed for recombinant, full-length, human transcription factors E2f1, E2f3, and E2f4. Briefly, universal PBMs involved binding of GST-tagged transcription factors to double-stranded 44K Agilent microarrays containing a DNA library designed to cover all possible 10-bp sequences, with every 8-mer occurring in at least 16 different spots on the array. This design allows comprehensive and unbiased characterization of the binding specificity of transcription factors for all possible 8-bp sequences.

Repositories


GEO

GSE117349

BioProject

PRJNA481797

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Contact


Raluca Gordan