|Dataset type:||Genome binding/occupancy profiling by array, Epigenomics|
|Number of samples:||3|
|Release date:||Jul 19 2018|
|Last update date:||Oct 19 2018|
|Dataset link||Divergence in DNA specificity among paralogous transcription factors contributes to their differential in vivo binding [uPBM_E2f1E2f3E2f]|
Universal protein-binding microarray (PBM) experiments were performed for recombinant, full-length, human transcription factors E2f1, E2f3, and E2f4. Briefly, universal PBMs involved binding of GST-tagged transcription factors to double-stranded 44K Agilent microarrays containing a DNA library designed to cover all possible 10-bp sequences, with every 8-mer occurring in at least 16 different spots on the array. This design allows comprehensive and unbiased characterization of the binding specificity of transcription factors for all possible 8-bp sequences.