Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 11
Release date: Apr 6 2015
Last update date: Aug 14 2018
Access: Public
Dataset link A transcriptional map following the developmental trajectory of the Arabidopsis stomatal lineage

Experimental Protocol


To obtain pure cell populations corresponding to different stomatal lineage cell types we used Fluorescent Activated Cell Sorting (FACS) in combination with markers of early (SPCHp::SPCH-YFP, SSY), middle (MUTEp::nucGFP, MG), and late (FAMAp::GFP-FAMA, FGF) precursor stages, as well of mature stomata (enhancer trap E1728::GFP, E1728G) and a marker of the entire epidermis (ML1p::YFP-RCI2A, ML1Y). To minimize transcriptional differences due to age, all cell types were sorted from 10-day old aerial rosettes, and the specificity of expression pattern at this common time point confirmed via confocal microscopy. Total RNA was extracted from purified protoplasts (4,000 to 20,000 cells/replicate; 2 replicates/marker line; 3 replicates/SSY marker line) and transcript abundance measured using RNA sequencing.

Repositories


GEO

GSE58856

ArrayExpress

E-GEOD-58856

ENA

SRP043607

BioProject

PRJNA253731

Download