Computational protocol: Temporal distribution of genetically homogenous ‘free-living’ Hematodinium sp. in a Delmarva coastal ecosystem

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Protocol publication

[…] The partial 18 S rRNA gene sequences obtained from the clone libraries were carefully checked for chimeric artifacts using the BLASTN program (http://www.ncbi.nlm.nih.gov/BLAST) and chimeric sequences were excluded from the phylogenetic analysis. Sequence alignment and phylogenetic reconstruction were performed using MEGA 5.05 software []. […]

Pipeline specifications

Software tools BLASTN, MEGA
Application Phylogenetics
Organisms Callinectes sapidus
Diseases Infection, Neural Tube Defects