Computational protocol: Mining of simple sequence repeats in the Genome of Gentianaceae

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Protocol publication

[…] A pair of primer flanking each SSR was designed using FastPCR software available at, which takes input according to user-defined conditions and pick primers according to these specified parameters. Default parameters of the FastPCR, viz, the optimum primer size of 20.0 (the range was 18–28), the optimum annealing temperature of 60.0 (the range was 57.0–63.0), and the range of% GC content of 44–60, were selected for primer designing. […]

Pipeline specifications

Software tools FastPCR, Primer3
Application qPCR