Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 23
Release date: Jun 23 2010
Last update date: Mar 21 2012
Access: Public
Computational protocol: GenePix Pro, limma, Pvclust, FigTree, GEO
Dataset link Location-specific responses to thermal stress in larvae of the reef-building coral Montastraea faveolata

Experimental Protocol


The experimental setup followed a reference design, i.e. all samples were hybridized against the same pool made up of equal amounts of RNA from all samples collected in Mexico. For samples from Mexico we used three technical replicates for each treatment temperature, for samples from Florida three biological replicates were used for each treatment temperature, except for the high temperature samples at day two where only two replicates were available due to high larval mortality at that temperature. Common reference samples were labeled with Cy3, temperature treatment samples with Cy5. Microarrays for M. faveolata contained 1,314 coding sequences, of which 43% had functional annotations as determined by homology to known genes.

Repositories


GEO

GSE19998

ArrayExpress

E-GEOD-19998

BioProject

PRJNA120165

Download


Contact


Nicholas Polato

Dataset Statistics

info

Citations per year

Dataset publication