Dataset features

Specifications


Application: sRNA-seq analysis
Number of samples: 2
Release date: Dec 7 2016
Last update date: Mar 12 2017
Access: Public
Diseases: Deficiency Diseases, Magnesium Deficiency
Chemicals: Amino Acids, Magnesium
Dataset link MicroRNA regulatory mechanisms on Citrus sinensis leaves to magnesium-deficiency

Experimental Protocol


Two libraries were constructed from Citrus sinensis (L.) Osbeck leaves subjected to 0(0 mM MgSO4,Mg-deficient,LD) or 1(Mg-sufficient,control, LS) mM MgSO4 for 16 weeks, and sequenced by Illumina sequencing. Size fractionated small RNAs (16-30 bp) from total RNA extracts was ligated to 5' and 3' adapters, and reverse transcribed. After PCR amplification the sample was subjected to Solexa sequencing. The clean tags were used for further analysis.

Repositories


GEO

GSE75758

ENA

SRP067031

BioProject

PRJNA305283

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Contact


Chen Lisong

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